GK107 ScienCell 人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit
ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。
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GK107 ScienCell人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit,產(chǎn)品簡介:
產(chǎn)品品牌: ScienCell
產(chǎn)品貨號:GK107
產(chǎn)品名稱:GK107 ScienCell人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK107 ScienCell人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit, 產(chǎn)品說明:
ScienCell的GeneQuery™人內(nèi)皮素信號傳導(dǎo)qPCR陣列試劑盒(GQH-EDN)調(diào)查了參與內(nèi)皮素信號轉(zhuǎn)導(dǎo)的88個關(guān)鍵基因,特別關(guān)注內(nèi)皮素-1。 內(nèi)皮素是調(diào)節(jié)血管收縮的分泌蛋白,主要在內(nèi)皮中產(chǎn)生。 內(nèi)皮素受體也存在于中樞神經(jīng)系統(tǒng)的細胞中,異常信號傳導(dǎo)可能與癲癇發(fā)作和周圍shenjingbing變有關(guān)。 與異常內(nèi)皮素信號傳導(dǎo)有關(guān)的其他疾病包括心血管疾病、血管痙攣、心臟肥大和登革熱。 如何對所包含的基因進行分類的簡要示例如下所示:
鈣調(diào)節(jié): CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
細胞骨架重塑:CAPN2,MAP2K1,PLD1,PLD2,RHOA
細胞周期調(diào)節(jié):CCND3、CDC42、ITPR1、ITPR3、RIPK1
轉(zhuǎn)錄因子:ELK1,FOS,JUN,MYC,SP1
RAS超家族GTPases:HRAS,KRAS,NRAS,RAC1,RAP1A
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設(shè)計的引物確保:(i)qPCR分析中的最佳退火溫度為65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。
ScienCell's GeneQuery™ Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1. Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium. Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy. Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever. Brief examples of how included genes may be categorized are shown below:
· Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
· Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA
· Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1
· Transcription factors: ELK1, FOS, JUN, MYC, SP1
· RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ 人內(nèi)皮素信號傳導(dǎo) qPCR 陣列板布局*(8 個粗體和斜體對照)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ADCY1 | CACNA1D | CDC42 | EDNRA | GSK3B | MAP2K1 | MYC | PIK3R1 | PPP1CA | PTGS2 | RIPK1 | β-actin |
B | ADCY3 | CALM1 | CGA | EDNRB | HBEGF | MAP3K1 | NOS3 | PIK3R2 | PPP3CC | PTK2B | SHC1 | GAPDH |
C | ADCY8 | CALM2 | CRK | EGFR | HRAS | MAPK13 | NPPB | PIK3R3 | PRKCA | RAC1 | SOS1 | LDHA |
D | AKT1 | CAMK2A | DGKB | ELK1 | ITPR1 | MAPK14 | NRAS | PLA2G4A | PRKCD | RAF1 | SOS2 | NONO |
E | AKT2 | CAMK2B | DGKE | FOS | ITPR2 | MAPK3 | NTRK1 | PLA2G7 | PRKCE | RAP1A | SP1 | PPIH |
F | ASIC1 | CAMK2D | ECE1 | GNAI1 | ITPR3 | MAPK9 | PIK3CA | PLCG1 | PTGER2 | RAPGEF1 | SRC | GDC |
G | ATF4 | CAPN2 | EDN1 | GNAQ | JUN | MMP2 | PIK3CB | PLD1 | PTGER4 | RASGRP1 | STAT5A | PPC |
H | CACNA1C | CCND3 | EDN3 | GRB2 | KRAS | MT-CO2 | PIK3CD | PLD2 | PTGS1 | RHOA | TRPV1 | NTC |
提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新
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